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CD11b+Ly6C++Ly6G- cells show distinct function in mice

The suppressive activity of the CD11b(+) cell populations from different donors was studied in co-culture experiments. 2012-12-12 · In contrast, mainly the CD11b + Ly6C + G + population, and to a lower extent the CD11b + Ly6C ++ Ly6G-population, expanded in spleen of mice with experimental autoimmune encephalomyelitis (EAE). The myeloid cells expanding during this chronic inflammatory condition could be regarded as MDSCs[ 11 ], since they potently suppressed T cell proliferation[ 22 ]. Markers such as CD11b, CD11c, F4/80, Gr-1, Ly6C, and Ly6G have long been used to identify various splenic cell myeloid populations. Flow cytometry and fluorescence-activated cell sorting (FACS) analysis demonstrated that Ly6G/Ly6C markers are superior to Gr-1 for identifying splenic neutrophils, eosinophils, and subsets of monocytes/macrophages. Methods: CD11b+Ly6C++ and Ly6G+ cells were isolated from spleen, tumor tissue or inflammatory granulomas. S100A9, Arginase 1 and iNOS gene expression in the various CD11b+ cell populations was analyzed using Q-PCR.

The CD11b + /Ly6C med /Ly6G + myeloid cells represent the granulocytic subset of a heterogeneous class of myeloid cells termed myeloid-derived suppressor cells (MDSCs; ref. 16). The monocytic fraction (CD11b + /Ly6C high /Ly6G −) suppresses CD8 + T cell and NK cell function, but the role of the granulocytic fraction is still unclear (16). CD11b + Ly6G − Ly6C hi cells, on the contrary, are the most prominent cell type at >3 d after CFA injection and are mostly present in the skin between 24 h and 3 d after incision.

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anti-Ly6C antibodies, which divide CD11b+Gr-1+ MDSCs into Ly6G + granulocytes and Ly6C monocytes (33). These two subpopulations may have different functions in infectious diseases and cancer34-36). Ly6C (hi monocytes have been reported to function as transient accessory cells to enhance angiogenesis and remodeling of existing small vessels into C57BL/6 mouse bone marrow stained with Ly-6G/Ly-6C APC (clone RB6-8C5, filled histogram) or APC Rat IgG2b, κ isotype control (open histogram).

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C) Representative plots from d 2 of data presented in B. D) Fold change (shown as log2) of the percentage of Ly6G + or Ly6C + of CD11b + cells in murine livers of Hx vs. sham. We observed that MPA promoted the accumulation of NK cells in spleens of tumor-bearing mice, but with reduced degranulation ability and in vivo cytotoxic activity. Simultaneously, MPA induced a preferential expansion of CD11b + Ly6G + Ly6C int cells in spleen and bone marrow of 4T1 tumor-bearing on CD11b +Gr1 -gated cells. Flow cytometry Single-cell suspensions were stained for 30 min at 4°C with the following antibodies: FITC anti-CD45, phycoerythrin anti-CD11b, allophycocyanin (APC) anti-Gr1, APC anti-Ly6G (IA8 clone), peridinin-chlorophyll protein (PerCP) Cy5.5 anti-Ly6C, APC anti-CD11c (BioLegend, San Diego, CA, USA), APC anti- In mice, monocytes are commonly identified as CD11b + F4/80 + CD115/M-CSF R + cells.

S100A9, Arginase 1 and iNOS gene expression in the various CD11b(+) cell populations was analyzed using Q-PCR. The suppressive activity of the CD11b(+) cell populations from different donors was studied in co-culture experiments.
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Hos möss MDSC uttrycker Mac-1 (CD11b) och Gr-1 (Ly6G och Ly6C) ytantigener 7. I musmodeller av metastaserande bröstcancer, kännetecknas en distinkt befolkning av makrofager som F4/80högLy6G–CD11bhögLy6Clåg developing bone marrow macrophages; including CD11b CD31 CD44 Ly6C Ly6G F4/80 Mac2 c-Fos as well as c-Fms. In addition RNA was preserved during that the delivery of CpG ODN directly into the tumor bed reduces the immunosuppressive activity of monocytic (CD11b(+), Ly6G(-), Ly6C(high)) MDSC. av M Kläschen · 2019 — CD11b är en integrin som framförallt uttrycks på makrofager och monocyter, Ly6C uttrycks framförallt på monocyter, Ly6G är unikt för neutrofiler, CD11c är ett skiljde sig åt mellan grupperna. CD11b är en integrin som framförallt uttrycks på makrofager och monocyter, Ly6C uttrycks framförallt på monocyter, Ly6G är Bland CD11b + -cellerna gated på F4 / 80-uttryck, Ly6G int Ly6C - var den dominerande befolkningen expanderad genom LPS-behandling, där Ly6G int av L Dieterich · 2011 · Citerat av 2 — particular, a population of CD11b+Gr-1+ immature myeloid cells appears to play an Ly6G and Ly6C, which are expressed by different subsets of immature and. Pac blue CD45.

Composition of total CD11b + cells, Ly6G + Ly6C lo granulocytic cells, and Ly6C hi monocytic cells in spleen (A) and bone marrow (B) of non-tumor-bearing mice with and without 8 … on CD11b +Gr1 -gated cells. Flow cytometry Single-cell suspensions were stained for 30 min at 4°C with the following antibodies: FITC anti-CD45, phycoerythrin anti-CD11b, allophycocyanin (APC) anti-Gr1, APC anti-Ly6G (IA8 clone), peridinin-chlorophyll protein (PerCP) Cy5.5 anti-Ly6C, APC anti-CD11c (BioLegend, San Diego, CA, USA), APC anti- Expression of uNK and CD11b + Ly6G hi Ly6C lo and CD11b + Ly6G lo Ly6C hi cell populations were analyzed using a flow cytometer. GMCSF treatment resulted in no significant change in the NK1.1 - DX5 - DBA + cell population from the CBA mice (p = 0.1164). However, an increase of the CD11b + Ly6G lo Ly6C hi population was observed (p = 0.0592). 65 Ly6G Primary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation. The myeloid differentiation antigen Gr-1 consists of two epitopes, recognized by anti-lymphocyte antigen (Ly) 6G and anti-Ly6C antibodies, which divide CD11b + Gr-1 + MDSCs into Ly6G + granulocytes and Ly6C + monocytes . These two subpopulations may have different functions in … Although Ly6C med−high monocytes constitute roughly 3~10-fold less numerous than circulating neutrophils during the initial 3~5 days after CVB3 infection, CD11b + Ly6G − Ly6C high/med monocyte and macrophage lineages comprise the major population of cardiac infiltrates in the later phase of CVB3 myocarditis (Fairweather et al., 2005; Huber, 2016).
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Anti-F4/80. MicroBeads. UltraPure. CD8+ Dendritic Cell Isolation Kit Ly6C+/–.

CD11b(+)Ly6C(++)Ly6G(-) cells show distinct function in mice with chronic inflammation or tumor burden. Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift On CD11b+ cells, they are expressed on Ly6C(hi) monocytes, neutrophils, and eosinophils (to start with).
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Tumör-associerade makrofagers inverkan vid - CORE

Markers such as CD11b, CD11c, F4/80, Gr-1, Ly6C, and Ly6G have long been used to identify various splenic cell myeloid populations. Flow cytometry and fluorescence-activated cell sorting (FACS) analysis demonstrated that Ly6G/Ly6C markers are superior to Gr-1 for identifying splenic neutrophils, eosinophils, and subsets of monocytes/macrophages. The CD11b + /Ly6C med /Ly6G + myeloid cells represent the granulocytic subset of a heterogeneous class of myeloid cells termed myeloid-derived suppressor cells (MDSCs; ref. 16). The monocytic fraction (CD11b + /Ly6C high /Ly6G −) suppresses CD8 + T cell and NK cell function, but the role of the granulocytic fraction is still unclear (16). CD11b + Ly6G − Ly6C hi cells, on the contrary, are the most prominent cell type at >3 d after CFA injection and are mostly present in the skin between 24 h and 3 d after incision. Ly6C med myeloid cells follow a pattern similar to that of Ly6C low cells after CFA injection, but have the same recruitment pattern as Ly6C hi cells after incision.

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Evaluation of in vitro macrophage differentiation during space flight

Markers such as CD11b, CD11c, F4/80, Gr-1, Ly6C, and Ly6G have long been used to identify various splenic cell myeloid popula-tions. Flow cytometry and fluorescence-activated cell sorting (FACS) analysis demon-strated that Ly6G/Ly6C markers are superior to Gr-1 for identifying splenic neutrophils, We observed that MPA promoted the accumulation of NK cells in spleens of tumor-bearing mice, but with reduced degranulation ability and in vivo cytotoxic activity. Simultaneously, MPA induced a preferential expansion of CD11b + Ly6G + Ly6C int cells in spleen and bone marrow of 4T1 tumor-bearing B) Percentage of CD11b + Gr1 + cells of total CD45 + cells isolated from murine livers following Hx or sham laparotomy, as determined by flow cytometry on indicated PODs.